bullet Sensors & Transducers Journal

    (ISSN 1726- 5479)


2008 e-Impact Factor

25 Top Downloaded Articles

Best Selling Articles 2012

Journal Subscription 2013

Editorial Calendar 2013

Submit an Article

Editorial Board

Current Issue

Sensors & Transducers journal's cover

Sensors & Transducers Journal 2011

Sensors & Transducers Journal 2010

Sensors & Transducers Journal 2009

Sensors & Transducers Journal 2008

Sensors & Transducers Journal 2007

2000-2002 S&T e-Digest Contents

2003 S&T e-Digest Contents

2004 S&T e-Digest Contents

2005 S&T e-Digest Contents

2006 S&T e-Digest Contents


Best Articles 2011




Vol. 146, No. 11, November 2012, pp. 78-89




Amperometric Acetylcholinesterase Biosensor Based on Poly (Diallyldimethylammonium Chloride)/Gold Nanoparticles/Multi-walled Carbon Nanotubes-chitosans Composite Film-modified Electrode
* Xia Sun, Zhili Gong, Yaoyao Cao, * Xiangyou Wang

School of Agriculture and Food Engineering, Shandong University of Technology,
No. 12, Zhangzhou Road, Zibo 255049, Shandong Province, P.R. China

Tel.:+86-533-2786558, fax: +86-533-2786558

E-mail: sunxia2151@sina.com, wxy@sdut.edu.cn


Received: 26 September 2012   /Accepted: 23 November 2012   /Published: 30 November 2012

Digital Sensors and Sensor Sysstems


Abstract: A novel amperometric biosensor was developed for the detection of organophosphorus pesticides (OPs). Acetylcholineesterase (AChE) was immobilized onto poly(diallyldimethylammonium chloride) (PDDA)/gold nanoparticles (AuNPs)/multi-walled carbon nanotubes-chitosans (MWCNTs-CHIT) composite film modified glass carbon electrode (GCE). The biosensor had good sensitivity owing to the excellent properties of MWCNTs and AuNPs, which promoted electron transfer and improved the electrochemical response. PDDA with positive charge was bound electrostatically to AChE with negatively charge by layer-by-layer (LbL) self-assembly technique, which can increase AChE loading amount. The morphologies and the sensitive steps of surface modification were characterized by scanning electron microscopy (SEM), cyclic voltammetric (CV) and electrochemical impedance spectroscopy (EIS), respectively. Based on the inhibition of OPs on the AChE activity, under the optimized conditions, the biosensor exhibited a linear response to chlorpyrifos in the concentration range from 0.8 to 20 μg/L and from 20 to 300 μg/L, with a detection limit of 0.56 g/L. The biosensor exhibited good reproducibility and acceptable stability. Moreover, PDDA-AChE/AuNPs/MWCNTs- CHIT/GCE biosensor can also be used for direct analysis of practical samples, which may open a new door for pesticide analysis in vegetables and fruits.


Keywords: Acetylcholinesterase, Multiwall carbon nanotubes, Gold nanoparticles, Poly (diallyldimethylammonium chloride), Biosensor



Buy this article online (it will be send to you in the pdf format by e-mail) or subscribe Sensors & Transducers journal

(12 issues per year plus special issues; 40 % discount for payment IFSA Members):



Sensors & Transducers journal subscription

450 $ US per year:


Buy this article for
14.95 $ US:





Alternatively we accept a money transfer to our bank account. Please contact for details: sales@sensorsportal.com


Download <here> the Library Journal Recommendation Form




Read more about Nanosensors and Biosensors






1999 - 2012 Copyright , International Frequency Sensor Association (IFSA). All Rights Reserved.

Home - News - Links - Archives - Tools - Voltage-to-Frequency Converters - Standardization - Patents - Marketplace - Projects - Wish List - e-Shop - Sensor Jobs - Membership - Videos - Publishing - Site Map - Subscribe - Search

 Members Area -Sensors Portal -Training Courses - S&T Digest - For advertisers - Bookstore - Forums - Polls - Submit Press Release - Submit White Paper - Testimonies - Twitter - Facebook - LinkedIn