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Vol. 164, Issue 2, February 2014, pp. 6-24




Recent Advance of Electrochemical Immunosensor for Pesticide Residues Detection

Lu QIAO, Huiying JIA, * Xia SUN, * Xiangyou WANG

School of Agriculture and Food Engineering, Shandong University of Technology, No. 12, Zhangzhou Road, Zibo 255049, Shandong Province, P.R. China
Tel.: +86-533-2786558, fax: +86-533-2786558

E-mail: sunxia2151@sina.com, wxy@sdut.edu.cn


Received: 4 December 2013 /Accepted: 28 January 2014 /Published: 28 February 2014

Digital Sensors and Sensor Sysstems


Abstract: Electrochemical immunosensors have emerged as a highly sensitive and rapid technique for the detection of specific pesticide based on the immunological interactions between the binding biomolecules (antibody or hapten) onto the transducer interface and the target analyte (hapten or antibody). In terms of the development of electrochemical immunosensors, the antibody (Ab) /hapten immobilization onto a transducer or a support matrice is a crucial step in improving the analytical performance, such as response, reproducibility, stability, selectivity and regeneration. This paper presents an overview of different types of electrochemical immunosensors and their principles and various immobilization protocols used for formation of a biorecognition interface, such as physical adsorption, covalent coupling, entrapment, oriented immobilization, self-assembled monolayer, nanoparticles. In addition, future prospects toward the development of electrochemical immunosensor systems are discussed.


Keywords: Electrochemical immunosensor, Pesticide residues, Immobilization protocols.


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