A Portable Pesticide Residues Detection Instrument Based on Impedance Immunosensor

School of Agriculture and Food Engineering, Shandong University of Technology, No. 12, Zhangzhou Road, Zibo 255049, P. R. China
Tel.: +86-533-2786558, fax: +86-533-2786558

Received: 7 April 2014 /Accepted: 30 May 2014 /Published: 30 June 2014

Abstract: In this paper, a design of portable pesticide residues detection instrument was presented based on an impedance immunosensor. The immunosensor exploited the novel multilayer films based on Au nanoparticles (AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes- chitosan nanocomposite (PANI/MWCNTs/CS). The detection principle of the instrument was based on the electrochemical characteristic of antigen-specific antibody immune response. With a stronger signal generated from the antigen-specific antibody immune response, the signal detection circuit was designed more easily. We integrated immunosensor and signal detection circuit to fabricate pesticide residues detection instrument. This proposed instrument could realize the rapid detection of pesticide residues in fruits and vegetables with automatic data processing and presented the result on the spot. The impedance test error was less than 5 %. The results showed that the proposed instrument had a good consistence compared with the traditional analytical methods. Thus, it would be a promising rapid detection instrument for pesticide residues in agricultural products.

Keywords: Pesticide residues, Immunosensor, Impedance detection circuit, Portable instrument, Antigen-specific antibody immune response.

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