Vol. 87, Issue 1, January 2008, pp.11-18

Kinetics Analysis of Respiratory Epithelium by Virtual Instrumentation

Libor HARGAŠ, Dušan KONIAR, Miroslav HRIANKA, Anna PRÍKOPOVÁ

Department of Mechatronics and Electronics,

Faculty of Electrical Engineering, University of Žilina,

Univerzitná 1, 010 26, Žilina, Slovak republic

E-mail: Libor.Hargas@fel.uniza.sk

Received: 20 December 2007   /Accepted: 21 January 2008   /Published: 28 January 2008

Abstract: The article deals with measurement and analysis of kinetic parameters of human respiratory epithelium. The article shows possibilities of data acquisition (videosequences) and postprocessing using virtual instrumentation – LabVIEW. The designed methods enable analysis and measurement of two parameters: cilia beat frequency (CBF) and trajectory of cilia of respiratory epithelium. The problem task is divided into videosequence acquisition of cilia from the microscope, region of interest (ROI) selection for analyzed data reduction, preprocessing of reduced sequences. Frequency analysis is done by intensity method, which records and evaluates intensity variations in ROI. Trajectory analysis uses sophisticated algorithms of object detection in the image called pattern matching. Results of this work are used for diagnostics of some pathology of respiratory apparatus and epithelium. All solution steps are realized in LabVIEW development system.

Keywords: Cilia, Respiratory epithelium, Virtual instrumentation, LabVIEW, Pattern matching

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