bullet Sensors & Transducers Journal

    (ISSN: 2306-8515, e-ISSN 1726-5479)


   About this Journal

   Information for Authors

   Editorial Board

   Editorial Calendar

   Current Issue

   Browse Journal

S&T journal's cover

Submit Press Release

Submit White Paper

25 Top Downloaded Articles (2007-2012)


Contact Us





Vol. 28, Special Issue, April-May 2018, pp. 38-42




Bacteriophage-modified Graphene Oxide Screen-printed Electrodes for the Impedimetric Biosensing
of Salmonella Enterica Serovar Typhimurium

1, * Pauline Ann QUITON, 3 Beatrice Marie CARREON, 1, 2, 4 Donna May DELA CRUZ-PAPA and 1, 2, 3 Jose BERGANTIN, Jr.

1 The Graduate School
2 Research Center for the Natural and Applied Sciences
3 Department of Chemistry
4 Department of Biology, College of Science, University of Santo Tomas, Espaňa Blvd., Manila 1015, Philippines
E-mail: pamquiton@gmail.com


Received: 28 March 2018 /Accepted: 28 May 2018 /Published: 31 May 2018

Digital Sensors and Sensor Sysstems


Abstract: In this study, an electrochemical impedance biosensor was developed using an isolated bacteriophage as a bioreceptor to detect Salmonella enterica serovar Typhimurium. The bacteriophage was immobilized on graphene oxide- modified screen-printed electrode (GO-SPE) using 1-(3-dimethylaminopropyl)- ethylcarbodiimide hydrochloride (EDC) as a crosslinker. The modified GO-SPEs were characterized using Field Emission Scanning Electron Microscopy and Fourier Transform Infrared Spectroscopy to confirm the successful immobilization of the bacteriophage on the electrode surface. Electrochemical Impedance Spectroscopy (EIS) was used to monitor variations in the charge transfer resistance (Rct) values at different immobilization and measurement conditions. The fabricated GO-SPE/Phage biosensor displayed quantitative response for S. Typhimurium over a broad range of concentrations (10-1 108 cfu/mL) and an increasing linear response was observed (R2=0.9842). The optimized incubation time and response time were 30 min and 4 min, respectively. Selectivity tests showed that the biosensor is selective towards S. Typhimurium over other bacteria, such as Escherichia coli and Staphylococcus aureus.


Keywords: Biosensors, Bacteria detection, Bacteriophage, Electrochemical Impedance Spectroscopy, Salmonella, Screen-printed electrode.


Acrobat reader logo Click <here> or title of paper to download the full pages article in pdf format



This work is licensed under a Creative Commons 4.0 International License


 Creative Commons License


Read more about Chemical and Biosensors






1999 - 2018 Copyright , International Frequency Sensor Association (IFSA) Publishing, S.L. All Rights Reserved.

Home - News - Links - Archives - Tools - Voltage-to-Frequency Converters - Standardization - Patents - Marketplace - Projects - Wish List - e-Shop - Sensor Jobs - Membership - Videos - Publishing - Site Map - Subscribe - Search

 Members Area -Sensors Portal -Training Courses - S&T Digest - For advertisers - Bookstore - Forums - Polls - Submit Press Release - Submit White Paper - Testimonies - Twitter - Facebook - LinkedIn