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Vol. 223, Issue 7, July 2018, pp. 1-8

 

Bullet

 

Development and Selection of Aluminum Screen Printed Amperometric Biosensor Modified
with Nanoparticles and Catalase Enzyme
 

1 Miriam Barquero-Quirós and 2 Marίa Julia Arcos-Martίnez

1 Department of Chemistry, University of Costa Rica, CELEQ, San Pedro de Montes de Oca, San José 11501-2060, Costa Rica
2 Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Ba?uelos s/n, Burgos 09001, Spain
1 Tel.: +506-2511-2442, fax: +506-2511-2446

E-mail: miriam.barquero@ucr.ac.cr, jarcos@ubu.es

 

Received: 16 May 2018 /Accepted: 5 July 2018 /Published: 31 July 2018

Digital Sensors and Sensor Sysstems

 

Abstract: Two biosensors were developed based in aluminum quantitative inhibition on gluthathione peroxidase and catalase enzymes. Screen printed electrode of gold was modified with gold nanoparticles and catalase enzyme. Screen printed carbon electrode was modified with gold nanoparticles; and glutathione peroxidase enzyme. Biosensors experimental conditions were optimized and characterized trough Michaelis Menten apparent constant. Inhibitory aluminum effect was confirmed by increasing constant apparent values with Al(III) concentration. Kinetic study for glutathione peroxidase resembles a competitive inhibition, while catalase showed competitive inhibition. Michaelis Menten apparent inhibition constant of catalase was lower than glutathione peroxidase biosensor and also its slope inhibition calibration curve was higher, therefore catalase biosensor was validated by its performance parameters. Catalase biosensor showed a detection limit of (1.0 ± 0.1) mM and quantification limit of (3.4 ± 0.3) mM. The repeatability was 8.3 % (n=3) and reproducibility was 10.7 % (n=5). Accuracy was tried spiking two concentrations of Al(III) standard reference material traceable to NIST. Recovery spiked concentrations of aluminum certified reference standard was 103.1 ± 5.3 (n=4) and 105.9 ± 4.4 (n=4).

 

Keywords: Aluminum, Screen printed carbon electrodes, Gold screen printed electrode, Catalase glutathione peroxidase inhibition, Validation.

 

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