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Vol. 27, Special Issue, May 2014, pp. 15-21




A Flexible Label-Free Biosensor Sensitive and Selective to TNF-a: Application for Chronic Heart Failure

1 Abdoullatif BARAKET, 1 Michael LEE, 1 Nadia ZINE, 2 Nourdine YAAKOUBI, 3 Maria Giovanna TRIVELLA, 4 Abdelhamid ELAISSARI,

1 Institut des Sciences Analytiques (ISA), Université Lyon, Université de Claude Bernard Lyon 1, UMR 5280, 5 rue de la Doua, 69100 Villeurbanne, 6 France
2 Université du Maine UMR CNRS 6613, rue Olivier Messiaen, 72085, LE MANS Cedex 9, France
3 CNR (Consiglio Nazionale Ricerche) Clinical Physiology Institute, Pisa, Italy
4 Université de Lyon, Université Claude Bernard Lyon-1, CNRS, UMR 5007, LAGEP, F-69622 Villeurbanne, France

E-mail: a.baraket@gmail.com, abdelhamid.errachid@univ-lyon1.fr


Received: 31 December 2012 /Accepted: 22 November 2013 /Published: 26 May 2014

Digital Sensors and Sensor Sysstems


Abstract: Tumor necrosis factor-a (TNF-a) is a key pro-inflammatory cytokine that is characterized by elevated circulating levels for chronic heart failure (CHF) and left ventricular assisted device (LVAD) implantation patients, respectively. Therefore, a rapid and ease-of-use diagnostic tool is required to monitor LVAD patients at a high risk of mortality during early expression of an inflammatory storm. In this paper, we report on the quantitative electrochemical detection of human TNF-a with its corresponding antibody (Ab) immobilized onto the functionalized biosensor surface. The label-free biosensor was fabricated on a gold surface that was deposited on a flexible polyimide (PI) substrate. The interfacial properties of the functionalized flexible gold electrodes were evaluated by cyclic voltammetry (CV) in the presence of Fe(CN)64-/3- as the redox-active species. Afterwards, the electrochemical impedance spectroscopy (EIS) technique was used to determine the TNF-a) concentrations. EIS results confirmed that the developed flexible biosensor can accurately detect TNF-a with a good sensitivity in the dynamic range of 0.1 pg/mL to 0.5 ng/mL. Overall, the developed flexible biosensor was easy to fabricate and the results demonstrate a good selectivity in the presence of other cytokines such as interleukin: (IL-10) and (IL-1).


Keywords: Tumor necrosis factor-a), Flexible gold electrodes, Electrochemical impedance spectroscopy.


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