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Vol. 27, Special Issue, May 2014, pp. 39-46




Adsorption Kinetics of Glutathione Monolayers on Platinum and Gold Surfaces
used for Ca2+ Sensor Development

1 Ferdaous MAATOUK, 1 Houcine BARHOUMI, 1 Abderrazak MAAREF and 2 Nicole JAFFREZIC-RENAULT

1 Laboratory of Interfaces and Advanced Materials (LIMA), University of Monastir 5000, Tunisia
2 Laboratory of Analytical Sciences, UMR CNRS 5180, University of Claude Bernard-Lyon1, Bâtiment Raulin, 69622 Villeurbanne Cedex, France.
1 Tel.: + (216) 73 500 278, fax: + (216) 73 500 280

E-mail: Houcine.Barhoumi@fsm.rnu.tn


Received: 31 December 2012 /Accepted: 10 August 2013 /Published: 26 May 2014

Digital Sensors and Sensor Sysstems


Abstract: This work investigates the electrochemical study of glutathione self-assembled monolayers (SAMs) prepared from aqueous solution on gold and platinum surfaces. The deposit SAMs monolayers were characterized by cyclic voltammetry and electrochemical impedance spectroscopy methods. In addition, the interpretation of electrochemical impedance spectroscopy results was based on the charge-transfer reaction using potassium hexacyanoferrate (II/III) as redox-active probe. The structure and the properties of the deposit monolayers on the metal surfaces were obtained under optimal experimental conditions. It was shown that the adsorption kinetics is also influenced by some parameters such as the metal nature, the temperature, the pH and the immersion time in the thiol solution. The developed self-assembled monolayer (SAM-Glutathione) on the gold surface was applied to the detection of calcium ions. As a result, a stable and reproducible calcium sensor was obtained with a sensitivity of 58.4 KW/pCa in the linear range from
10-4 M to 10-1 M. A detection limit around 10-4 M for Ca2+ ions was obtained.


Keywords: Gold and Platinum Surfaces, SAM-Glutathione, Impedance Spectroscopy, Calcium Sensor.


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