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Vol. 27, Special Issue, May 2014, pp. 127-136




Immunosensors Based on Single-Walled Carbon Nanotubes (SWCNT) for the Detection
of Deep Venous Thrombosis

1, 2, 3 Sondes BOURIGUA, 2 Abderrazak MAAREF, 1 Abdelhamid ERRACHID, 1 François BESSUEILLE, 1, * Nicole JAFFREZIC-RENAULT

1 University of Lyon, Laboratory of Analytical Sciences, UMR-CNRS 5180, Claude Bernard University, Lyon I, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
2 University of Monastir, Laboratory of Adanced Materials and Interfaces, Faculty of Sciences of Monastir: Avenue of environnement, 5019 Monastir Tunisie
3 Laboratoire Méthodes et Techniques d'Analyses-INRAP, 2020-Ariana Tunisie
*Tel.: +33472431182, fax: +33472431206

E-mail: Nicole.jaffrezic@univ-lyon1.fr


Received: 31 December 2012 /Accepted: 10 August 2013 /Published: 26 May 2014

Digital Sensors and Sensor Sysstems


Abstract: Thanks to their properties, Single-Walled carbon nanotubes (SWNT) open a new way to the fabrication of Immunosensors with the particularity to amplify the response signal from antibody–antigen interaction and to improve the Immunosensors characteristics. In this context, two new impedimetric immunosensors were developed by immobilizing antibody on Single-Walled carbon, the later was immobilized following two ways the first consist of immobilizing the carbon nanotubes on a polypyrrole layer by adsorption and the second consist of functionalized gold with amino thiol and then immobilizing the carbon nanotubes with covalent binding. The electrical properties and the morphology of the immunosensors have been characterized respectively by Electrochemical Impedance Spectroscopy, cyclic voltammetry and Atomic Force Spectroscopy. A low detection limit for both immunosensors was determined as 1 pg/ml and linear ranges up to 10 ng/ml with polypyrrole and up to 100 ng/ml with amino thiol were obtained. Moreover, the studied Immunosensors exhibited high sensitivity, stability and reproducibility.


Keywords: D-dimer, Carbon nanotubes, Impedance spectroscopy, Polypyrrole, Amino thiol.


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