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Vol. 27, Special Issue, May 2014, pp. 165-169




Microconductometric Detection of Bacterial Contamination

1, 2 Sarra EL ICHI, 1 Fanny LEON, 1 Ludivine VOSSIER, 3 Hélène MARCHANDIN, 2 Abdelhamid ERRACHID, 2* Nicole JAFFREZIC-RENAULT, 1 Joliette COSTE, 1 Chantal FOURNIER-WIRTH, 4 Jan KREJČÍ, 4 Radka KUČEROVÁ, 4 Tomáš KREJČÍ

1 Etablissement Français du Sang, Laboratoire TransDiag, Parc Euromédecine, 392 Avenue Professeur Jean-Louis Viala, 34184 Montpellier, France
2 Université de Lyon, Institut des Sciences Analytiques, UMR CNRS 5280, 5 Rue de La Doua, 69100 Villeurbanne, France
3 CHRU Montpellier, Laboratoire de Bactériologie UMR 5119, Université Montpellier 2, Place Eugéne Bataillon, 34095 Montpellier, France
4 BVT Technologies a.s, Hudcova 78c, 61200Brno, Czech Republic
1 Tel.: +33437423558

E-mail: nicole.jaffrezic@univ-lyon1.fr


Received: 31 December 2012 /Accepted: 10 August 2013 /Published: 26 May 2014

Digital Sensors and Sensor Sysstems


Abstract: Several approaches can be used for the electrochemical detection of bacterial contamination. Their performance can be assessed by the ability to detect bacteria at very low concentrations within a short-time response. We have already demonstrated that a conductometric biosensor based on interdigitated thin-film electrodes is adapted to detect bacteria in clinical samples like serum and compatible with microfluidic fabrication. The type of interdigitated microelectrodes influences the performance of the biosensor. This was shown by the results obtained in this work. A magnetic-nanoparticles based immunosensor was designed using gold screen-printed electrodes. The immunosensor was able to specifically detect E. coli in the range of 1-103 CFU mL-1. The new transducer offered a larger active sensing surface with a lower cost and a robust material. Accuracy of the conductance value was enhanced by differential measurements. The immunosensor is compatible with a microfluidic system.


Keywords: Bacteria, Interdigitated electrodes, Immunosensor, Conductometry.


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