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Vol. 27, Special Issue, May 2014, pp. 312-318




Tapered Optical Fiber Sensor for Detection of pH in Microscopic Volumes


Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, v.v.i., Chaberska 57, 182 51 Prague 8, Czech Republic
Tel.: +420 266 773 558, fax: +420 284 680 222

E-mail: podrazky@ufe.cz


Received: 23 November 2013 /Accepted: 12 January 2014 /Published: 26 May 2014

Digital Sensors and Sensor Sysstems


Abstract: A compact and robust tapered optical fiber microsensor is presented for detection of pH in a range from 5.8 to 7.5 in sub-microliter volumes. The sensor is based on a pH transducer 8- hydroxypyrene-1,3,6-trisulfonic acid trisodium salt immobilized in a xerogel matrix onto the tip of a optical fiber taper with a tip diameter below 20 mm. The sol-gel method and two silicon alkoxides is used for preparing the matrix. A ratio of the fluorescence emission intensities measured at 518 nm after the excitation at 400 and 450 nm is used for evaluating the sensor response to pH. This ratiometric approach enables to reduce effects of ambient light, bleaching of the sensitive layer and geometry of the probe to the fluorescence signal and achieve the resolution of about 0.07 pH units.


Keywords: Optical sensor, Ph, Microfiber tip, Fluorescence, HPTS, Ratiometrc method.


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