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Vol. 27, Special Issue, May 2014, pp. 361-365




Characterisation by Impedance Spectroscopy and Capacitance-Voltage of an EMIS Sensor Functionalized by Catalase for Nitrite Detection


1 Université de Jijel, BP 98, Ouled Aissa, 18000 Jijel, Algeria
2 Université de Lyon - LSA - UMR 5180 CNRS - Université Claude Bernard Lyon 1, 69622 Villeurbanne cedex, France
Tel.: +213 774000720

E-mail: azazoua@yahoo.fr


Received: 23 November 2013 /Accepted: 12 January 2014 /Published: 26 May 2014

Digital Sensors and Sensor Sysstems


Abstract: Impedance spectroscopy and capacitance-voltage (C-V) methods are a rapidly developing electrochemical technique for the characterization of biomaterial–functionalized electrodes and biocatalytic transformations on the electrodes surface, and specifically for the transduction of biosensing events at electrodes. Such techniques have been used in our work as a tool for the characterization of a new nitrite biosensor for environmental applications based on the immobilization of catalase on insulator-semiconductor (IS) systems (p-Si/SiO2/Si3N4). The principle of the developed biosensor includes the following: Catalase catalyzed the breakdown of H2O2 into H2O and O2. Nitrite was selected as an inhibitor of catalase. Under optimal conditions, i.e. buffer capacity corresponding to 3 mM phosphate buffer, the catalase enzyme insulator semiconductor sensors shows a high sensitivity to nitrite detection. In both cases, the responses of these biosensors based on nitrite additions are good with the detection limit around 10-11 M. It is expected that such an original and promising concept of inhibitor-based biosensors based on reactivation by inhibitive effects, will be useful for the development of environmental smart biosensors based on the integration of ENFET with the corresponding instrumentation in the same silicon chip.


Keywords: EIS structure, Biosensors, Catalase, Nitrite, EMIS, Electrochemical measurement.


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