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Vol. 27, Special Issue, May 2014, pp. 371-373

 

Bullet

 

Immobilization of Candida Rugosa Lipase on Aluminosilicate Incorporated in a Polymeric Membrane
for the Elaboration of an Impedimetric Biosensor
 

1 Nedjla ZEHANI, 1 Rochdi KHERRAT, 2 Nicole JAFFREZIC-RENAULT

1 Laboratory of Environmental Engineering, Department of Process Engineering, Faculty of Engineering Sciences, Badji Mokhtar University -Annaba,
B. P. 12, Annaba, 23000, Algeria
2 University of Lyon, Institute of Analytical Chemistry,
5 Rue de La Doua, 69100 Villeurbanne, France

E-mail: Nedjlaz@yahoo.fr

 

Received: 23 November 2013 /Accepted: 12 January 2014 /Published: 26 May 2014

Digital Sensors and Sensor Sysstems

 

Abstract: This work describes the development of a new biosensor for the detection of an organophosphate insecticide (Diazinon) in an aqueous medium. The method is based up on the immobilization of Candida Rugosa Lipase enzyme by adsorption on Aluminosilicate in order to incorporate it into a polymeric membrane. The electrical properties of the polymeric membrane have been characterized by electrochemical impedance spectroscopy (EIS). The linear range of the biosensor is from 10-8 M to 10-6 M with a detection limit of 10-8 M.

 

Keywords: Immobilization, Aluminosilicate, Biosensor, Diazinon, Impedance.

 

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