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Vol. 148, No. 1, January 2013, pp. 89-94




A Portable Colloidal Gold Strip Sensor for Clenbuterol and Ractopamine Using Image Processing Technology
1 Yi Guo, 1 Feng He, 1,* Youquan Zhao, 2 Yanjun Fang, 2 Zhuge Xi

1 College of Precision Instrument and Opto-electronics Engineering, Tianjin University,

92 Weijin Road, Nankai District, Tianjin, P. R. China 300072

Tel.: 86-13820413401

2 Institute of Hygiene and Environmental Medicine, Academy of Military Medical Science,

1 Dali Road, Heping District, Tianjin, P. R. China 300050

Tel.: 86-13920838035

E-mail: zhaoyouquan@tju.edu.cn, yanjunfang1973@163.com


Received: 2 September 2012   /Accepted: 24 January 2013   /Published: 31 January 2013

Digital Sensors and Sensor Sysstems


Abstract: A portable colloidal golden strip sensor for detecting clenbuterol and ractopamine has been developed using image processing technology, as well as a novel strip reader has achieved innovatively with this imaging sensor. Colloidal gold strips for clenbuterol and ractopamine is used as first sensor with given biomedical immunication reaction. After three minutes the target sample dropped on, the color showing in the T line is relative to the content of objects as clenbuterol, this reader can finish many functions like automatic acquit ion of colored strip image, quantatively analysis of the color lines including the control line and test line, and data storage and transfer to computer. The system is integrated image collection, pattern recognition and real-time colloidal gold quantitative measurement. In experiment, clenbuterol and ractopamine standard substance with concentration from 0 ppb to 10 ppb is prepared and tested, the result reveals that standard solutions of clenbuterol and ractopamine have a good secondary fitting character with color degree (R2 is up to 0.99 and 0.98). Besides, through standard sample addition to the object negative substance, good recovery results are obtained up to 98 %. Above all, an optical sensor for colloidal strip measure is capable of determining the content of clenbuterol and ractopamine, it is likely to apply to quantatively identifying of similar reaction of colloidal golden strips.


Keywords: Biosensor, Portable strip reader, Image processing, Clenbuterol, Ractopamine


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