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Vol. 92, Issue 5, May 2008, pp. 134-143




Magnetoelastic Biosensor Design: an Experimental Study of Sensor Response and Performance


Rajesh GUNTUPALLI1,3*, Ramji S. LAKSHMANAN1, Jiehui Wan1, Z-Y. CHENG1, Vitaly J. VODYANOY3, Bryan A. CHIN1

1 Materials Research and Education center, Auburn University, Auburn, AL-36849, USA

2 Department of Nutrition and Food Science, Auburn University, Auburn, AL- 36849, USA

3 Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL- 36849, USA

* Corresponding author. Tel.: +1 334 844 3322; E-mail: guntura@auburn.edu (R. Guntupalli)



Received: 21 March 2008   /Accepted: 20 May 2008   /Published: 26 May 2008


Abstract: This article presents the results of an investigation into the development and characterization of polyclonal antibody immobilized magnetoelastic biosensors.  Langmuir-Blodgett (LB) monolayer technique was employed for antibody (specific to Salmonella. sp) immobilization on rectangular shaped strip magnetoelastic sensors.  Biosensor performance was investigated by exposing to graded concentrations (5×101 through 5×108 cfu/ml) of Salmonella typhimurium solutions in a flow through mode.  Bacterial binding to the antibody on the sensor surfaces changed the resonance parameters, and these changes were quantified by the sensor’s resonance frequency shift.  Biosensor response towards S. typhimurium was measured in different liquid samples (water, fat free milk and apple juice) and as well as in the presence of extraneous microorganisms (Escherichia coli O157:H7 and Listeria monocytogenes). Biosensor performance was characterized by evaluating mechanical merit factor (Q-value) in different liquids, specificity towards various bacterial species and also the thermal stability of biosensor was investigated at different temperatures 25, 45 and 65 °C.


Keywords: Magnetoelastic biosensor, Salmonella typhimurium, Q-value, specificity, longevity, Sensitivity


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