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Vol. 239, Issue 12, December 2019, pp. 1-8

 

Bullet

 

Detection of Aflatoxin B1 Using an Immunosensor Based on CF/AuNPs Composite Membrane
and Normalized Sweep Potential Voltammetry
 

1, 2, 3, * Wenping Zhao, 1 Ya Zhao, 1 Jianfei Sun, 1, 2, 3 Yemin Guo and 1, 2, 3, * Xia Sun

1 School of Agriculture Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zhangdian District, Zibo City, 255000, Shandong Province, P.R. China
2 Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zhangdian District, Zibo City, 255000, Shandong Province, P.R. China
3 Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zhangdian District, Zibo City, 255000, Shandong Province, P.R. China
1 Tel.: +86-533-2786558, fax: +86-533-2786558

E-mail: 187286528@qq.com, sunxia2151@sina.com

 

Received: 6 November 2019 /Accepted: 30 November 2019 /Published: 5 December 2019

Digital Sensors and Sensor Sysstems

 

Abstract: A novel electrochemical immunosensor was prepared for rapid detection of aflatoxin B1 (AFB1) using experimental data processed by normalized sweep potential voltammetry (NSPV).Carbon fiber (CF) and gold nanoparticles (AuNPs) had been coated on gold (Au) electrodes as sensitive composite membrane to prepare sensor for AFB1 detection. The immunoreaction monitoring was achieved by cyclic voltammetry (CV) techniques and the immunoreaction currents were analyzed by NSPV. According to different normalized values, a better electrode type was selected (Au), and the optimized types of electrode-modified materials (CF and AuNPs), modification sequence (AuNPs/CF), pH of PBS (7.4), BSA concentration (2.5%), and antibody concentration (10 g/mL) were determined. The best detection voltage (0.11 V) was obtained by normalized processing. With the optimal conditions met, the linearity between peak currents of the incubated electrochemical biosensor and AFB1 concentrations was investigated, and the results indicated a standard curve, which showed an excellent linear relationship within the concentration range of 0.01 to 1000 ng/mL. The linear regression formula for the concentrations of AFB1 versus the currents was y = 0.12967 + 0.03774x (R2 = 0.98858), while the limit of detection (LOD) for this method was 0.01 ng/mL. The recovery results showed that the sensor had high specificity and could be used for rapid detection of AFB1 residues in rice.

 

Keywords: Electrochemical immunosensor, AFB1, Gold nanoparticles, Carbon fiber, Normalized sweep potential voltammetry (NSPV).

 

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