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Vol. 239, Issue 12, December 2019, pp. 9-16

 

Bullet

 

Preparation of Electrochemical Immunosensor for Aflatoxin B1 Detection and Normalizing Methods
Used for Experiment Parameter Optimization
 

1, 2, 3, * Wenping Zhao, 1 Hui Zhang, 1 Jianfei Sun, 1, 2, 3 Yemin Guo, 1, 2, 3, * Xia Sun and 1 * Zhonghe Liu

1 School of Agriculture Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zhangdian District, Zibo City, 255000, Shandong Province, P.R. China
2 Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zhangdian District, Zibo City, 255000, Shandong Province, P.R. China
3 Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zhangdian District, Zibo City, 255000, Shandong Province, P.R. China
1 Tel.: +86-533-2786558, fax: +86-533-2786558

E-mail: 187286528@qq.com, sunxia2151@sina.com, 364989694@qq.com

 

Received: 6 November 2019 /Accepted: 30 November 2019 /Published: 1 December 2019

 

Digital Sensors and Sensor Sysstems

 

Abstract: We proposed a sensitive electrochemical immunosensor for rapid aflatoxin B1 (AFB1) detection based on gold nanoparticles (AuNPs) and carbon nanofibers (CNFs) modified electrodes. The modification of nanoparticles and the fixation of biomolecules on the electrodes had an influence on electron transfer on the electrode surface, causing changes in response currents. The response current was tested through cyclic voltammetry (CV) techniques and the current changes were analyzed by normalization method. Experiment parameters were optimized according to the response current changes on electrodes. According to the results of the normalization processing, the best parameters for the biosensor construction were determined and these parameters included the electrode type (Au), electrode-modified materials and its modification sequence (AuNPs/CF), pH of PBS (7.4), BSA concentration (2.5 %) and antibody concentration (10 g/mL). Under optimized conditions, we constructed an immune sensor for aflatoxin detection and the testing results indicated that the sensor had good performance, which provided a new idea for rapid aflatoxin detection.

 

Keywords: Immunosensor, Aflatoxin B1, Rapid detection, Parameter optimization, Normalization methods.

 

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