Vol. 107, Issue 8, August 2009, pp. 111-118

Epinephrine Biosensor Using Tyrosinase Immobilized Eggshell Membrane

^{a, b} Sanket Tembe, ^a Sudha Kulkarni, ^a,1Meena Karve, ^{b, 2}S. F. D’Souza

^a Department of Chemistry, University of Pune, Ganeshkhind, Pune 411 007, India

^b Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre,

Mumbai, 400 085, India

²Tel.: + 91 22 25593632, fax: + 91 22 25505151

¹Tel.: + 91 20 25601401, fax: + 91 20 25691728

E-mail: sfdsouza@apsara.barc.ernet.in, mskarve@chem.unipune.ernet.in

Received: 2009   /Accepted:  August 2009   /Published:  August 2009

Abstract: Electrochemical biosensor based on immobilized tyrosinase was developed for the determination of epinephrine. The enzyme was extracted from a plant source Amorphophallus companulatus and immobilized on eggshell membrane. Tyrosinase catalyses oxidation of epinephrine to epinephrinequinone which is electrochemically reduced at -0.17 V versus Ag/AgCl (3 M KCl). The resulting current was used for epinephrine quantification. The sensor showed linearity in the range 3 x 10^-5 to 3 x 10^-4 M with a detection limit of 1 x 10^-5 M. It has reusability upto 15 cycles and a shelf life of more than 6 months when stored at 4 ^o C.

Keywords: Tyrosinase, Epinephrine, Biopolymers, Electrochemical biosensor

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